Biolegend, 508801, Biotin anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody, 50μg

The C8.6 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C8.6 antibody has been reported to strongly inhibit different biological activities of IL-12, (e.g., IFN-gamma induction, mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity). The C8.6 antibody can neutralize the bioactivity of natural or recombinant IL-12.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: COS-expressed p40 subunit of human IL-12
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection, ELISPOT Detection
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Neutralization1,2,3: The LEAF™Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-12 bioactivity (Cat. No. 508804).
Additional Product Notes: ELISA or ELISPOT Detection1: The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p40 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the C8.3 antibody (Cat. No. 501702/501704) as the capture antibody. The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified 7B12 antibody (Cat. No. 511002/511004) as the capture antibody. Each lot of this antibody is quality control tested by ELISA assay.
Application References(PubMed link indicates BioLegend citation): D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. Goodier M, et al. 2000. J. Immunol. 165:139. Aragane Y, et al. 1994. J. Immunol. 153:5366.
Product Citations: Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed Haug V, et al. 2020. Transplantation. e260:104. PubMed Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
RRID: AB_315528 (BioLegend Cat. No. 508801) AB_315529 (BioLegend Cat. No. 508802)
Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL
Receptors: IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 3593
UniProt: View information about IL-12/IL-23 p40 on UniProt.org
Clone: C8.6
Regulatory Status: RUO
Other Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.