Biolegend, 511202, Purified anti-human IL-23 (p19) Antibody, 500μg

The HLT2736 clone recognizes human IL-23, a heterodimeric cytokine composed of the p40 subunit shared with IL-12 and a specific p19 subunit. This antibody recognizes heterodimeric IL-23, but does not react with the p40 subunit alone or recombinant IL-12(p70) when used as a capture antibody in ELISA. IL-23 is secreted by activated dendritic cells and macrophages and has been shown to enhance IFN-γ secretion by memory (CD45RO) T cells in an IL-2 dependent manner. IL-23 may also induce unique Th subset (designated Th IL-17 ) that secretes the cytokines IL-17, IL-6, TNF and low levels of IFN-γ. IL-23 has been shown to promote immunity to mycobacteria and has been shown to be upregulated in response to some viral infections and in psoriatic lesions. IL-23 binds to the IL-23 receptor comprised of a β1 subunit shared with the IL-12 receptor and IL-23-specific subunit. This antibody has been shown to be useful for Western blotting and immunohistochemistry using formalin-fixed, paraffin embedded tissues with (catalyzed signal amplification).
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human recombinant p40 and p19 with elastin linker
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting applications, a concentration range of 0.5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The purified HLT2736 antibody has been shown to be useful for Western blotting and immunohistochemistry using formalin-fixed, paraffin embedded tissues (with catalyzed signal amplification). This antibody weakly stains frozen sections. This clone does not cross-react with mouse.
Application References(PubMed link indicates BioLegend citation): Doherty SD, et al. 2006. Arch. Dermatol. Res. 298(7):353-6. Ohyama H, et al. 2009. J. Dent. Res. 88:633. (IHC-P) PubMed Katsifis GE, et al. 2009. Am. J. Pathol. 175:1167. (IHC-P) PubMed Schlapbach C, et al. 2011. J. Am. Acad. Dermatol. 65:790. PubMed
Product Citations: Katsifis G, et al. 2009. Am J Pathol. 175:1167. PubMed Ohyama H, et al. 2009. J Dent Res. 88:633. PubMed Li H, et al. 2018. Nat Commun. 9:2830. PubMed Tripodo C, et al. 2010. Am J Pathol. 177:792. PubMed Bohnacker S, et al. 2020. Mol Immunol. 120:126. PubMed Schlapbach C, et al. 2011. J Am Acad Dermatol. 65:790. PubMed Mideksa YG, et al. 2022. J Biol Chem. :102677. PubMed
RRID: AB_416323 (BioLegend Cat. No. 511201) AB_416324 (BioLegend Cat. No. 511202)
Structure: Member of the IL-12 cytokine family, heterodimeric cytokine composed of p40 subunit shared with IL-12 and a specific p19 subunit
Distribution: Secreted by activated dendritic cells and macrophages
Function: Enhances IFN-γ secretion by memory (CD45RO) T cells in an IL-2 dependent manner; may induce unique Th subset (designated ThIL-17) secreting IL-17, IL-6, TNF and low levels of IFN-γ in EAE model; shown to promote immunity to mycobac
Ligand/Receptor: IL-23 receptor (β1 subunit of IL-12 receptor and IL-23-specific subunit)
Cell Type: Dendritic cells, Macrophages
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Parham C, et al. 2002. J. Immunol. 168:5699. 2. Verreck FA, et al. 2004. Proc. Natl. Acad. Sci. USA 101:4560. 3. Lee E, et al. 2004. J. Exp. Med. 199:125. 4. Oppmann B, et al. 2000. Immunity 13:715.
Regulation: Upregulated in viral infections such as HSV, increased in psoriatic lesions in monocytes and mature dendritic cells
Gene ID: 51561
UniProt: View information about IL-23 p19 on UniProt.org
Clone: HLT2736
Regulatory Status: RUO
Other Names: p19p40, JKA3 induced upon T cell activation
Isotype: Mouse IgG1, κ