Biolegend, 512305, PE anti-human IL-17A Antibody, 25tests

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4 + Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4 + helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-17A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed Corbière V, et al. 2023. Front Immunol. 14:1101366. PubMed Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed Donadei C, et al. 2023. J Clin Med. 12:. PubMed Hu X, et al. 2022. EBioMedicine. 86:104333. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Sun L, et al. 2023. Mol Med Rep. 27: . PubMed Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed Tankou SK, et al. 2018. Ann Neurol. 83:1147. PubMed Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed Wu B, et al. 2020. Cell Metabolism. 32(6):967-980.e5. PubMed Chruewkamlow N, et al. 2016. PLoS One. 11: 0145983. PubMed Fraccaroli L, et al. 2015. J Leukoc Biol. 98: 49 - 58. PubMed Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Du Y, et al. 2022. Nat Commun. 13:231. PubMed Tomalka J, Jesus T, Ramakrishnan P 2016. J Leukoc Biol. 100: 111 - 120. PubMed Gabrion A, et al. 2017. J Allergy Clin Immunol. 139:1641. PubMed Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed Lichnog C, et al. 2019. Front Pharmacol. 10:39. PubMed Hr TN, et al. 2021. Allergol Immunopathol (Madr). 49:193. PubMed Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed Lambert EE, et al. 2020. Vaccines (Basel). 8:. PubMed
RRID: AB_961395 (BioLegend Cat. No. 512305) AB_961394 (BioLegend Cat. No. 512306)
Structure: Cytokine; dimer; ~15 kD (Mammalian)
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: Activated T cell subpopulation
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41. 2. Furuzawa-Carballeda J, et al. 2007. Autoimmun. Rev. 6:169. 3. Witowski J, et al. 2007. Kidney Int. 71:514. 4. Gaffen SL, et al. 2006. Vitam. Horm. 74:255. 5. Hymowitz S, et al. 2001. EMBO J. 20:5332.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: BL168
Regulatory Status: RUO
Other Names: Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.