Biolegend, 512701, Purified anti-human IL-17A Antibody, 50μg

IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63% and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-17A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.25 - 1.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-17A recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.
Additional Product Notes: ELISA Capture: The purified BL23 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5189 (Cat. No. 518902) antibody as the detection antibody and recombinant human IL-17A (Cat. No. 570509) as the standard.
Product Citations: Sy M, et al. 2023. J Neuroinflammation. 209:20. PubMed
RRID: AB_1027616 (BioLegend Cat. No. 512702)
Structure: Cytokine; dimer
Distribution: IL-17A is largely produced by activated memory T lymphocytes, CD4+ T helper cells (Th17), neutrophils, CD8(+), NK, and gamma-delta T cells (3).
Function: IL-17A is a potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Organ overexpression of IL-17A increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. Il-17A induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. IL-17A also cooperates with TLR ligands, IL-1 beta, and TNF alpha to enhance inflammatory reactions and stimulate production of beta-defensins and other antimicrobial peptides (1, 2).
Interaction: IL-17AR is expressed in epithelial cells, fibroblasts, B and T lymphocytes, myelomonocytic cells, marrow stromal cells, synovial endothelial cells and chondrocytes from arthritic patients express IL-17R (4)
Ligand/Receptor: IL-17A signals through a heteromeric receptor composed of IL-17RA and IL-17RC.
Bioactivity: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources: activated T cell subpopulation
Cell Targets: Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors: IL-17R
Cell Type: Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hirota K, et al. 2007. J. Exp. Med. 204:41. 2. Furuzawa-Carballeda J, et al. 2007. Autoimmun Rev. 6:169. 3. Witowski J, et al. 2007. Kidney Int. 71:514. 4. Gaffen SL, et al. 2006. Vitam Horm. 74:255. 5. Hymowitz S, et al. 2001. EMBO J. 20:5332.
Gene ID: 3605
UniProt: View information about IL-17A on UniProt.org
Clone: BL23
Regulatory Status: RUO
Other Names: IL-17, IL-17AF, IL-17A/F, Interleukin 17A, Interleukin 17AF, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype: Mouse IgG1, κ