Biolegend, 512902, Purified anti-mouse VEGF-A Antibody, 200μg

VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted glycoproteins that belongs to structurally related superfamily of dimeric growth factors with a cysteine knot motif. This superfamily includes platelet-derived growth factor (PDGF) and transforming growth factor (TGF) family. VEGF-A plays important roles in both vasculogenesis and angiogenesis, including tumor associated angiogenesis and vascular leakage. VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: N-terminal 24 aa sequence of murine VEGF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Immunohistochemistry: Clone 1F07-2C01 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655. Kirsch AH, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed.
Product Citations: Kutikhin AG, et al. 2020. Cells. :9. PubMed Kirsch A, et al. 2012. Am J Physiol Renal Physiol. 303:569. PubMed Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed Wang L, et al. 2020. Biomed Res Int. 2020:9485398. PubMed
RRID: AB_2212504 (BioLegend Cat. No. 512901) AB_2212503 (BioLegend Cat. No. 512902)
Structure: cystine-knot growth factors
Distribution: aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells
Function: angiogenesis
Cell Targets: endothelial cells
Receptors: VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Cell Type: Macrophages
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Contreras A, et al. 2007. Am. J. Transpl. 7:155. 2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109. 3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267. 4. Reinders MEJ, et al. 2003. J. Clin. Invest. 112:1655 5. Ferrara N,et al. 2003. Nat. Med. 9:669. 6. Shibuya M. 2001. Cell Struct. Funct. 26:25. 7. Senger DR, et al. 1983. Science 219:983.
Regulation: HIF-1, hypoxiaresponsiveness
Gene ID: 22339
UniProt: View information about VEGF-A on UniProt.org
Clone: 1F07-2C01
Regulatory Status: RUO
Other Names: Vascular endothelial growth factor (VEGF), VEGF-A, VEGFA
Isotype: Rat IgG, κ