Biolegend, 513001, Purified anti-human IL-21 Antibody, 50μg

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine primarily produced by NKT cells and CD4 + T cells, particularly the inflammatory Th17 subset. It exerts pleiotropic effects on both innate and adaptive immune responses. These include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. IL-21 also has proapoptotic effects on B cells and NK cells. Recent studies have shown that IL-21 functions as an autocrine cytokine, strongly inducing Th17 differentiation and suppressing Foxp3 expression, making it a potential therapeutic target for treating cancer and inflammatory diseases.
50μg
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant full length human IL-21
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-21 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Drutman SB, et al. 2012. J Immunol. 3603:188. PubMed Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed Schwabenland M, et al. 2021. Immunity. . PubMed Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
RRID: AB_1027620 (BioLegend Cat. No. 513001) AB_1027621 (BioLegend Cat. No. 513002)
Distribution: Th17
Function: Immunomodulation
Cell Sources: NK-T cells and T cells
Cell Targets: Cytotoxic T cells (CTL) and Natural Killer (NK) cells that can destroy malignant or infected cells
Receptors: IL-21R
Cell Type: Th17
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nurieva R. 2007. Nature 448:416. 2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856. 3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144. 4. Asao H, et al. 2001. J. Immunol. 167:1. 5. Parrish-Novak J, et al. 2000. Nature 408:57.
Gene ID: 59067
UniProt: View information about IL-21 on UniProt.org
Clone: 3A3-N2
Regulatory Status: RUO
Other Names: Interleukin 21
Isotype: Mouse IgG1, κ