Biolegend, 514402, Purified anti-mouse IL-25 (IL-17E) Antibody, 500μg

IL-25 (IL-17E) is a member of the IL-17 family of proinflammatory cytokines which include at least six members including IL-17A (IL-17), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. IL-17E is found at low levels in various peripheral tissues. It is a proinflammatory cytokine that promotes airway eosinophilia in mice. IL-17E plays a role in Th2 type inflammatory diseases, such as asthma and atopic dermatitis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-25
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA - Quality tested Neut - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Neutralization: The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-25 bioactivity (Cat. No. 514417).
Application References(PubMed link indicates BioLegend citation): Angkasekwinai P, et al. 2007. J. Exp Med. 204:1509.
RRID: AB_2043954 (BioLegend Cat. No. 514401)
Structure: Cytokine; 19 kD
Bioactivity: Induce proinflammatory responses, promote the expression of the prototypical Th2 genes
Cell Sources: Activated Th2 cells, bone marrow-derived mast cells, and alveolar macrophages
Cell Targets: IL-17RB mRNA transcripts are abundantly expressed in the kidney and liver and at lower levels in the testes, brain, small intestine, and other organs.
Receptors: IL-17BR (EVI27, IL17Rh1)
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fort MM, et al. 2001. Immunity 15:985. 2. Lee J, et al. 2001. J. Biol Chem. 276:1660. 3. Hurst SD, et al. 2002. J. Immunol. 169:443. 4. Kleinschel MA, et al. 2007. J. Exp. Med. 204:161.
Gene ID: 140806
UniProt: View information about IL-25 on UniProt.org
Clone: 35B
Regulatory Status: RUO
Other Names: IL-17E
Isotype: Rat IgG1, κ