Biolegend, 514604, FITC anti-human IL-8 Antibody, 100tests

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: ELISA Capture: The purified BH0814 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BH0840 (Cat. No. 514703/ 514704) antibody as the detecting antibody and recombinant human IL-8 (Cat. # 570909) as the standard.
Product Citations: Zang Y, et al. 2012. Clin Cancer Res. 18:5639. PubMed
RRID: AB_2126227 (BioLegend Cat. No. 514604)
Structure: CXC chemokine; 8.4 kD (Mammalian)
Ligand/Receptor: CXCR1 (CDw128); CXCR2
Bioactivity: Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
Cell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Cell Targets: Neutrophils, basophils, lymphocytes
Receptors: CXCR1 (CDw128); CXCR2
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97. 3. Schröder J, et al. 1992. Immunology Ser. 57:387. 4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.
Regulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-β, glucocorticoids, vitamin D3
Gene ID: 3576
UniProt: View information about IL-8 on UniProt.org
Clone: BH0814
Regulatory Status: RUO
Other Names: Interleukin-8, Neutrophil activating protein-1, Monocyte-derived neutrophil chemotactic factor (MDNCF), Neutrophil activating factor (NAF), Leukocyte adhesion inhibitor (LAI), Granulocyte chemotactic protein (GCP), CXCL8
Isotype: Mouse IgG2b, κ