Biolegend, 516409, APC anti-mouse IL-22 Antibody, 100tests

IL-22 is a cytokine structurally related to IL-10.  Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD.  It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.  It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells.  AhR activation increases Th17 polarization and induces IL-22 production.  IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ.  IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin.  IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease.  Moreover, it plays a critical role in mucosal immunity and the wound healing process.
100tests
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard. In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively. Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations. Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended. Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1
Application References(PubMed link indicates BioLegend citation): Veldhoen M, et al. 2009. J. Exp. Med. 206:43.
Product Citations: Rolla S, et al. 2015. Clin Sci. 130: 193 - 203. PubMed Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed Mihi B, et al. 2021. Cell Reports Medicine. 2(6):100320. PubMed Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed Di Pietro C, et al. 2016. J Immunol. 197: 3271 - 3280. PubMed Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID: AB_2563355 (BioLegend Cat. No. 516409)
Structure: A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Distribution: Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.
Function: Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing.
Cell Type: NK cells, Th1, Th17, Th2
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Martin B, et al. 2009. Immunity 31:321. 2. Liang SC, et al. 2006. J. Exp. Med. 203:2271. 3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43. 4. Zheng Y, et al. 2007. Nature 445:648. 5. Dumoutier L, et al. 2000. J. Immunol. 164:1814. 6. Wolk K, et al. 2007. J. Immunol. 178:5973.
Gene ID: 50929
UniProt: View information about IL-22 on UniProt.org
Clone: Poly5164
Regulatory Status: RUO
Other Names: Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF
Isotype: Goat Polyclonal IgG