Biolegend, 517901, Purified anti-mouse IFN-γ Antibody, 50μg

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IFN-γ protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested ELISPOT, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein (Cat. No. 575309) are recomended at concentration up to 2000 pg/mL. The final standard range should be determined by the end-user.. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detection antibody, and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Prat M, et al. 1984. P. Natl. Acad. Sci. USA 81:4515. (IP) Kort JJ, et al. 2006. J. Neuroimmunol. 173:23. (ELISPOT) PubMed Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations: Qin J, et al. 2023. Front Immunol. 14:1126392. PubMed Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed Adhikari AS, et al. 2022. Front Oncol. 12:850546. PubMed Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
RRID: AB_10900246 (BioLegend Cat. No. 517901) AB_10900809 (BioLegend Cat. No. 517902)
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
Gene ID: 15978
UniProt: View information about IFN-gamma on UniProt.org
Clone: AN-18
Regulatory Status: RUO
Other Names: IFN-gamma
Isotype: Rat IgG1, κ