Biolegend, 519105, PE anti-human IL-16 Antibody, 25tests

Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4 + T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.
25tests
Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.
Application References(PubMed link indicates BioLegend citation): Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC) Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA) Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC) Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC) Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
Product Citations: Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed
RRID: AB_10897940 (BioLegend Cat. No. 519106)
Structure: 12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution: Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.
Function: Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor: CD4.
Cell Type: Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Bellon M, et al. 2010. Blood 115:2441. 2. Rofani C, et al. 2009. Stem Cells Dev. 18:671. 3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467. 4. Gu Y, et al. 2008. J. Immunol. 181:4418. 5. McFadden C, et al. 2007. J. Immunol. 179:6439. 6. Qi JC, et al. 2006. Blood 107:135.
Gene ID: 3603
UniProt: View information about IL-16 on UniProt.org
Clone: 14.1
Regulatory Status: RUO
Other Names: Interleukin-16
Isotype: Mouse IgG2a
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.