Biolegend, 520004, LEAF™ Purified anti-human SCF Antibody, 100μg

Stem Cell Factor (SCF) was initially synthesized as membrane-bound protein of 248 or 220 amino acids, depending on alternative splicing of exon 6. The 248 amino acid form contains a proteolytic cleavage site encoded by exon 6 which is cleaved from the cell to release an active soluble protein of 165 amino acid residues. Soluble SCF is glycosylated at both N-linked and O-linked sites. MMP-9 plays a physiological role in SCF release from the membrane, and this action plays a significant role in differentiating and mobilizing stem and progenitor cells from the bone marrow. SCF increases the proliferation of myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF/c-kit interaction in mast cells results in mast cell degranulation with release of mediators, such as histamine and inflammatory cytokines and chemokines. Activation of c-kit in dendritic cells regulates T helper cell differentiation and allergic asthma. In addition, SCF plays an important role in re-vascularization of ischemic limbs. Ischemia induces plasma elevation of SCF and thrombopoietin (TPO) and, in lower levels, GM-CSF and erythropoietin (EPO). SCF and TPO can induce the release of CXCL12 from platelets into plasma. This results in an extensive mobilization of CXCR4+VEGFR1+ cells (hemangiocytes), accelerating re-vascularization of the ischemic limbs. SCF binds to its receptor kit that belongs to the type III tyrosine kinase family, whose members include receptors for M-CSF and PDGF.
100μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant human SCF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by the ability to block the binding of an experimentally-determined spike concentration of human CD117-Fc to immobilized human SCF (Cat. No. 573904) The ND50 range for this effect is 0.04 - 0.3 µg/mL. It is recommended that this reagent be titrated to confirm performance.
RRID: AB_3683469 (BioLegend Cat. No. 520004)
Structure: Homodimer
Distribution: Embryonic Stem Cells, Hematopoietic stem and progenitors
Function: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Interaction: Mast cells, natural killer cells, dendritic cells, eosinophils, epithelial cells, endothelial cells, melanocytes, germ cells, cholangiocytes, platelets, myeloid leukaemia cells, and intestinal cells of Cajal.
Ligand/Receptor: c-kit (CD117)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Lu HS, et al. 1996. J Biol Chem. 271:11309-16. Heissig B, et al. 2002. Cell. 109:625-37. Jin DK, et al. 2006. Nat. Med. 12:557-67. Krishnamoorthy N, et al. 2008. Nat Med. 14:565-73. Ray P, et al. 2010. Ann N. Y. Acad. Sci. 1183:104-22.
Gene ID: 4254
UniProt: View information about SCF on UniProt.org
Clone: Poly5200
Regulatory Status: RUO
Other Names: Stem cell factor, KIT-ligand, Kitl, KL-1, mast cell grow factor (MGF), steel factor (SF), FPH2, SHEP7
Isotype: Goat Polyclonal IgG
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.