Biolegend, 522503, Biotin anti-human VEGF Antibody, 50μg

VEGF (also known as VEGF-A) is a signaling protein with strong vascular permeability activity that stimulates the formation of new blood vessels during the processes of vasculogenesis and angiogenesis. It is a member of the vascular endothelial growth factor family, which in mammals is comprised of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). There are multiple isoforms of VEGF that result from alternative splicing of mRNA. VEGF binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), through which VEGF exerts its mitogenic effects. VEGF is highly expressed in solid tumors of breast, lung, renal, colorectal, and hepatic origin. Additionally, it significantly contributes to ascites tumor formation. Beyond cancer, VEGF has also been associated with multiple pathogenic conditions including rheumatoid arthritis, age-related macular degeneration, Crow-Fukase syndrome, sclerosis and Alzheimer’s disease.
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.1 - 1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of VEGF recombinant protein ranging from 23.4 to 1,500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The biotinylated Poly5225 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with purified A15136H antibody (Cat. No. 537002) as the detection antibody and Recombinant Human VEGF (ELISA Std.) (Cat. No. 560309) While Poly5225 was generated against VEGF-165 and is quality tested against VEGF-165, cross-reactivity to other isoforms of VEGF has not been determined.
RRID: AB_2783338 (BioLegend Cat. No. 522503)
Structure: Homodimer
Distribution: Widely expressed
Function: VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition VEGF is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells and plays important roles during wound healing. Also, VEGF regulates hematopoietic stem cell survival.
Interaction: VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages
Ligand/Receptor: Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1)
Antigen References: Conn G, et al. 1990 Proc. Natl. Acad. Sci USA 87:1323. Gerber H, et al. 2002. Nature 417:954. Shibuya M. 2006. J. Biochem. Mol. Biol. 39:469. Shibuya M. 2008. BMB Rep. 41:278. Monaghan-Benson E, et al. 2010. The American Journal of Pathology 177:2091. Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
Gene ID: 7422
UniProt: View information about VEGF on UniProt.org
Clone: Poly5225
Regulatory Status: RUO
Other Names: VEGFA, VEGF-A, VPF, MVCD1, vascular endothelial growth factor
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.