Biolegend, 524004, Biotin anti-mouse/rat/human MIF Antibody, 50μg

MIF (human macrophage migration inhibitory factor) is a non-glycosylated polypeptide expressed by multiple cell types, which include activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. It is secreted by response to diverse inflammatory stimuli, and has been associated with a clear pro-inflammatory and proatherogenic role in multiple studies involving patients and animals. MIF also positively correlates with obesity as well as insulin resistance and contributes to adipose tissue inflammation by modulating ATM functions. Furthermore, MIF has been described as a protein that plays an essential role in both innate and acquired immunity.
50μg
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MIF recombinant protein ranging from 10 to 0.16 ng/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
RRID: AB_2686961 (BioLegend Cat. No. 524004)
Structure: 115 amino acids with a predicted molecular weight of approximately 12.5 kD.Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.
Distribution: Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.
Function: MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
Interaction: Monocytes and macrophages, mesenchymal cells, and B-cells.
Ligand/Receptor: CD44/CD74, CXCR4, CXCR2, and CXCR7.
Cell Type: T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Zhou H, et al. 2009. PLoS One. 4:e6087. 2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889. 3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73. 4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737. 5. van der Vorst EP, et al. 2015. Front Immunol. 6:373. 6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161. 7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519. 8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1. 9. Bach JP, et al. 2008. Oncology 75:127.
Gene ID: 17319
UniProt: View information about MIF on UniProt.org
Clone: Poly5240
Regulatory Status: RUO
Other Names: Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF, Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase
Isotype: Goat Polyclonal IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.