Biolegend, 524104, PE anti-human CXCL5 Antibody, 100tests

CXCL5, also known as ENA-78, is a 11.9 kD chemokine and belongs to the intercrine alpha, CXC chemokine family. It is expressed by monocytes and eosinophils in response to IL-1 and TNFα. CXCL5 can interact with CXCR2 and activate neutrophils and induce their migration. In vitro neutrophil chemotactic activity of CXCL5 is three fold higher. IFNγ suppresses expression of CXCL5. NFκB alone is sufficient to induce CXCL5 in contrast to IL-8, which requires both NFκB and C/EBP. Two forms are produced by N terminal proteolytic cleavage, ENA78 (8-78) and ENA 78 (9-78), and these isoforms exhibit higher chemotactic activity. Increased intra-hepatic levels of CXCL5 have been reported in patients with chronic liver disease.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CXCL5 (ENA-78)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2562377 (BioLegend Cat. No. 524103) AB_2562378 (BioLegend Cat. No. 524104)
Structure: CXC chemokine family, 11.9 kD
Distribution: Monocytes, platelets, and eosinophils
Function: Interacts with CXCR2 and is involved in neutrophil activation and chemotaxis for HIV-1
Ligand/Receptor: CXCR2
Cell Type: Eosinophils, Monocytes, Platelets
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Walz A, et al. 1991. J. Exp. Med. 174:1355. 2. Power CA, et al. 1994. Gene. 151:333. 3. Corbett MS, et al. 1994. Biochem. Biophys. Res. Commun. 205:612.
Gene ID: 6374
UniProt: View information about CXCL5 on UniProt.org
Clone: J111B7
Regulatory Status: RUO
Other Names: ENA-78, ENA78, epithelial-derived neutrophil-activating protein 78, small inducible cytokine B5
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.