Biolegend, 525501, Purified anti-human MIF Antibody, 50μg

Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide expressed by multiple cell types, including activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles in biological processes such as catalytic activity, immunity, endocrine regulation, signal modulation, and inflammation. MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors. Studies suggest that MIF might serve as a molecular link between chronic inflammation and cancer. Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly dimeric and trimeric forms.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human MIF with a His tag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality testedWB - Verified
Recommended Usage: Each lot of this protein is quality control tested by ELISA assay. A concentration of 4 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.
Application Notes: ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody.Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.
Application References(PubMed link indicates BioLegend citation): Zhou H, et al. 2009. PLoS ONE. 4:e6087. (FA, Neut)
Product Citations: Pérez-de Puig I, et al. 2013. J Cereb Blood Flow Metab. 1955:33. PubMed
RRID: AB_2563133 (BioLegend Cat. No. 525501) AB_2563134 (BioLegend Cat. No. 525502)
Structure: Predominantly a homotrimer
Distribution: Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells
Function: Pro-inflammatory cytokine, regulates macrophage function, involved in cancer development, tautomerase activity
Interaction: Monocytes and macrophages, mesenchymal cells, B-cells
Ligand/Receptor: CD74, CXCR4, CXCR2, CXCR7
Cell Type: Endothelial cells, Eosinophils, Epithelial cells, Macrophages, T cells
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Zhou H, et al. 2009. PLoS ONE. 4:e6087. 2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889. 3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73. 4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
Gene ID: 4282
UniProt: View information about MIF on UniProt.org
Clone: 10C3
Regulatory Status: RUO
Other Names: Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF
Isotype: Mouse IgG2b, κ