Biolegend, 525602, Purified anti-human CCL28 (MEC) Antibody, 500μg

Human CCL28 was identified in an EST database using a human chemokine consensus sequence. CCL28 is constitutively expressed by mucosal epithelial cells such as exocrine glands, trachea, and colon. Acinar epithelial cells in human and mouse salivary glands are strongly positive for CCL28 by immunostaining, and human saliva and milk contain high concentrations of CCL28. Furthermore, it is known that human and mouse CCL28 have antimicrobial activity attributed to the C-terminus of CCL28 against Candida albicans , Gram-negative bacteria, and Gram-positive bacteria. In addition, using a CCR10 deficient mouse model, it was shown that CCR10 plays a critical role in localization and accumulation of IgA ASC in lactating mammary glands. Also, it has been described that CCL28 is upregulated in epithelial inflammation, and this upregulation allows the recruitment of Tregs expressing CCR10. In fact, a subpopulation of CCR10-expressing CD25+CD4+FOXP3+ Tregs with potent anti-inflammatory properties was isolated from a chronically inflamed human liver. Information suggests that tumor hypoxia promotes the recruitment of Tregs through induction of expression of CCL28, and this induction is mediated by the hypoxia inducible factor-1α (HIF-1α). Therefore, tumor hypoxia promotes tolerance and angiogenesis via CCL28 and Tregs.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CCL28
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL28 recombinant protein ranging from 1000 to 15.6 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified J132C3 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated J139G11 antibody (Cat. No. 525703) as the detection antibody.Note: For testing human CCL28 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CCL28 ELISA Kit with Pre-coated Plates (Cat. No. 441207 & 441208) are specially developed and recommended.
RRID: AB_2563257 (BioLegend Cat. No. 525602)
Structure: Chemokine
Distribution: Mucosal epithelial cells, primary human cholangiocytes
Function: CCL28 chemoattracts resting CD4, CD8, and IgA antibody secreting cells. It plays a role in extravasation of IgA ASC in the small intestine, colon, lactating mammary glands, and has antimicrobial activity. CCL28 is upregulated by IL-1 or n-butirate in human colon epithelial cell lines, and IL-1 and n-butirate have a synergistic effect on CCL28 upregulation. Hypoxia induces CCL28 in tumors.
Interaction: CD4 and CD8 T cells, IgA antibody secreting cells, CCR10-expressing CD25+ CD4+ Foxp3+ Tregs, CCR3 expressing eosinophils
Ligand/Receptor: CCR10, CCR3
Cell Type: B cells, Eosinophils, Epithelial cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Wang W, et al. 2000. J. Biol. Chem. 275:22313. 2. Hieshima K, et al. 2003. J. Immunol. 170:1452. 3. Hieshima K, et al. 2004. J. Immunol. 173:3668. 4. Ogawa H, et al. 2004. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G1062. 5. Eksteen B, et al. 2006. J. Immunol. 177:593. 6. Morteau O, et al. 2008. J. Immunol. 181:6309. 7. Liu B and Wilson E. 2010. Eur. J. Immunol. 40:186. 8. Facciabene A, et al. 2011. Nature 475:226.
Gene ID: 56477
UniProt: View information about CCL28 on UniProt.org
Clone: J132C3
Regulatory Status: RUO
Other Names: Mucosa-associated epithelial chemokine (MEC), CCK1, SCYA28, small inducible cytokine A28
Isotype: Mouse IgG2b, κ