Biolegend, 526002, Purified anti-human M-CSF Antibody, 500μg

M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells. It binds CD14 + monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts and, consequently, this makes M-CSF a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF production in human monocytes and human tumor cells. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human M-CSF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2563444 (BioLegend Cat. No. 526002)
Structure: Three isoforms with 256-554 amino acids, predicted molecular weight of approximately 30-70 kD.
Distribution: Cell membrane; single-pass membrane protein. Processed macrophage colony-stimulating factor 1: secreted, extracellular space.
Function: Controls the production, differentiation, and function of macrophages.
Interaction: Homodimer or heterodimer, disulfide-linked. Interacts with CSF1R.
Ligand/Receptor: M-CSFR or CSF1R (CD115).
Biology Area: Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Kawasaki ES, et al. 1985. Science 230:291. 2. Wei S, et al. 2010. J. Leuko. Biol. 88:495. 3. Hodge JM, et al. 2011. PloS One 6:e21462. 4. Morandi A, et al. 2011. PloS One 6:e27450. 5. Erblich B, et al. 2011. PloS One 6:e26317. 6. MacDonald KP, et al. 2010. Blood 116:3955.
Gene ID: 1435
UniProt: View information about M-CSF on UniProt.org
Clone: L1617F1
Regulatory Status: RUO
Other Names: CSF1, CSF-1, MCSF
Isotype: Mouse IgG2b, κ