Biolegend, 528601, Purified anti-human CCL8 (MCP-2) Antibody, 50μg

CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells that are stimulated with IL-1β. CCL8 (MCP-2) has a structure and function that is similar with both MCP-1 and MCP-3. It is often co-induced with those chemokines in mononuclear cells. IFNγ is a strong inducer of CCL8 (MCP-2) in monocytes and fibroblasts. CCL8 (MCP-2) in monocytes and fibroblasts and can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemo attract monocytes, but it binds to the CCR2 receptor and induces its internalization. As a result, CCL8 (MCP-2) inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 (MCP-2) is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of the Th2 homing, which drives IL-5 mediated chronic allergic inflammation. In addition, CCL8 (MCP-2) binds the CCR5 receptor with a high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 (MCP-2) is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.
50μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CCL8.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 – 2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 250 to 3.9 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified L3715B6 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5210 antibody as the detection antibody and recombinant human CCL8 (MCP-2) as the standard.Note: For testing human CCL8 (MCP-2) in serum, plasma, or supernatant, BioLegend’s LEGEND MAX™ Human CCL8 (MCP-2) ELISA Kit is specially developed and recommended.
RRID: AB_2565222 (BioLegend Cat. No. 528601) AB_2565225 (BioLegend Cat. No. 528602)
Structure: Chemokine.
Distribution: Fibroblasts, endothelial cells, monocytes, and macrophages.
Function: CCL8 (MCP-2) chemo attracts monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils. CCL8 (MCP-2) is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 (MCP-2) by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Interaction: Monocytes, T cells, natural killer (NK) cells, mast cells, eosinophils, and basophils.
Ligand/Receptor: CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
Cell Type: Endothelial cells, Fibroblasts, Macrophages, Monocytes
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Van Damme J, et al. 1992. J. Exp. Med. 176:59. 2. Proost P, et al. 1998. J. Immunol. 160:4034. 3. Dean RA, et al. 2008. Blood 112:3455. 4. Hori T, et al. 2008. Blood 111:4403. 5. Rom S, et al. 2010. FASEB J. 24:2292. 6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843. 7. Islam SA, et al. 2011. Nat. Immunol. 12:167.
Gene ID: 10635
UniProt: View information about CCL8 on UniProt.org
Clone: L3715B6
Regulatory Status: RUO
Other Names: HC14, MCP-2
Isotype: Mouse IgG1, κ