Biolegend, 531304, Biotin anti-human IL-1α Antibody, 50μg

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. Recombinant human IL-1α is an 18.0 kD protein containing 159 amino acids.
50μg
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Goat
Immunogen: Recombinant human IL-1α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA Detection - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.25 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
Application Notes: The biotinylated Poly5313 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified 343-3B3-14 (Cat. No. 500104) antibody as the capture antibody. Note: For human IL-1a in cell culture supernatant, serum, plasma or other bodily fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 434907 & 434908) are specially developed and recommended.
RRID: AB_2686960 (BioLegend Cat. No. 531304)
Structure: Cytokine
Distribution: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglias, gliomas, keratinocyte
Function: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Interaction: B cells, T cells, monocytes, dendritic cells
Ligand/Receptor: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Cell Type: Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C et al. 1996. Blood 87:2095.
Gene ID: 3552
UniProt: View information about IL-1alpha on UniProt.org
Clone: Poly5313
Regulatory Status: RUO
Other Names: Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)(il-1a,il1a)
Isotype: Goat Polyclonal Ig
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.