Biolegend, 569506, Biotinylated Recombinant Human CD47-Fc Chimera (carrier-free), 100μg

CD47 (Cluster of differentiation 47), also known as integrin-associated protein (IAP), is a ~50 kD glycosylated protein belonging to the immunoglobulin superfamily. It is a ubiquitously expressed integral membrane protein with a single IgV-like domain at its N-terminus, five membrane-spanning regions, and an alternatively spliced cytoplasmic C-terminus. CD47 was first identified as a protein involved in β3 integrin-mediated signaling in leukocytes. CD47 interacts with SIRPα (signal regulatory protein-alpha), TSP-1 (thrombospondin-1) and other integrin members to regulate cell migration, axon extension, cytokine production and T cell activation. CD47 binds to SIRP family members on macrophages, neutrophils and T cells. These interactions function as a negative immune checkpoint to prevent the macrophage-mediated clearance of healthy CD47-expressing cells. CD47 is a potent ‘do not eat me’ signal that enables cancer cells to evade detection by the innate immune system. Atherogenesis is associated with upregulation of CD47, a key antiphagocytic molecule that is known to render malignant cells resistant to programmed cell death. In several preclinical studies, therapeutic antibodies that block the interaction between CD47 and SIRPα promote effective antitumor responses by enabling elimination of both hematologic and solid tumor cells by phagocytes, enhancing cross-presentation of CD8 + T cells by antigen-presenting cells, and triggering adaptive immune responses.
100μg
Source: Human CD47-Fc Chimera, amino acid (Gln19-Pro139) (Accession: Q08722), with a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in CHO cells. Human CD47-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass: The 400 amino acid recombinant protein has a predicted molecular mass of approximately 44.6 kD. The DTT-reduced and non-reduced protein migrates at approximately 55-65 kD and 110-120 kD respectively in SDSPAGE.
N-terminalSequence Analysis: The predicted N-terminal amino acid is Gln.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.2 with 10% glycerol
Endotoxin Level: Less than 0.1 EU per μg of protein as determined by the LAL method
Concentration: 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Biotinylated recombinant human CD47-Fc Chimera binds to immobilized recombinant human SIRPα in dose-dependent manner. The ED50 for this effect is 2.5 – 15 ng/mL.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Monomer
Distribution: Expression on most cell types
Function: Regulates cell migration, axon extension, cytokine production and T cell activation and is involved in β3 integrin-mediated signaling
Interaction: Macrophages, neutrophils and T cells
Ligand/Receptor: SIRPα (signal regulatory protein-alpha), TSP-1 (thrombospondin-1) and other integrin members
Bioactivity: Biotinylated recombinant human CD47-Fc Chimera binds to immobilized recombinant human SIRPα in dose-dependent manner. The ED50 for this effect is 2.5-15 ng/mL
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts, Neurons, Platelets
Biology Area: Cell Adhesion, Immuno-Oncology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References: Wu AL, et al. 1999. Mol Cell. 4:619-25. Han X, et al. 2000. J. Biol. Chem. 275:37984-92. Oldenborg PA, et al. 2000. Science. 288:2051–2054. Latour S, et al. 2001. J Immunol. 167:2547-54. Piccio L, et al. 2005. Blood. 105:2421-7. Gardai SJ, et al. 2005. Cell. 123:321-34. Hatherley D, et al. 2008. Mol Cell. 31:266-77. Majeti R, et al. 2009. Cell. 138:286-299. Willingham SB, et al. 2012. Proc Natl Acad Sci USA. 109:6662-7. Liu X, et al. 2015. Nat Med. 21:1209-15. Weiskopf K, et al. 2016. Clin Invest.126:2610-20. Casey SC, et al. 2016. Science. 352:227-31. Kojima Y, et al. 2016. Nature. 536:86-90. Osorio JC, et al. 2023. Cancer Cell. 41:2051-2065.
Gene ID: 961
UniProt: View information about CD47 on UniProt.org
Regulatory Status: RUO
Other Names: Antigenic surface determinant protein OA3, Integrin-associatedprotein (IAP), Protein MER6