Biolegend, 570314, Cell-Vive™ GMP Recombinant Human IL-15 (carrier-free), 25μg

IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many similar functions as IL-2, for example, stimulating the proliferation of activated T cells, NK cells and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils, and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
25μg
Source: Human IL-15, amino acid Asn49-Ser162 (Accession # P40933), was expressed in E.coli.
Molecular Mass: The 114 amino acid recombinant protein has a predicted molecular mass of approximately 12.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 11 kD and 7 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Asn.
N-terminalSequence Analysis: Asn-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys
Purity: >95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 µm filtered protein solution is in PBS.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤ 0.500 ng/μg by ELISA
Concentration: 500 µg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Cell-Vive™ GMP Recombinant Human IL-15 induces the proliferation of MO7e megakaryocytic leukemic cells in a dose-dependent manner. The ED50 for this effect is 2 - 10 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human IL-15 (carrier-free) is ≥ 1.4 x 108 IU/mg when compared against an internal control. The internal control was calibrated against the WHO International Standard for Human IL-15 (NIBSC code: 95/554).
Application: Bioassay Cell Cultures
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: 13 kD non-glycosylated polypeptide
Distribution: IL-15 is expressed by DC, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes.
Function: IL-15 stimulates the proliferation of activated T cells, NK cells and B cells. IL-15 induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils, and induces the production of proinflammatory cytokines from macrophages.
Interaction: T lymphocytes, NK cells
Ligand/Receptor: IL-15Rα, IL-15Rβ and IL-15γc
Bioactivity: Measured by its ability to induce proliferation of MO7e human megakaryocytic leukemic cells
Cell Type: Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: Grabstein K, et al. 1994. Science. 264:965-8. Ma A, et al. 2006. Annu Rev Immunol. 24:657-79. Meresse B, et al. 2004. Immunity. 21:357-66. Armitage RJ, et al. 1995. J Immunol. 154:483-90. Pulendran B, et al. 2004. Eur J Immunol. 34:66-73. Ratthé C, et al. 2004. J Leuko Biol. 76:162-8. Bouchard A, et al. 2004. J Leuko Biol. 75 893-900. Tang F, et al. 2008. Cell Immunol. 5:189-96. Rubinstein MP, et al. 2006. Proc Natl Acad Sci U S A. 103:9166-71.
Gene ID: 3600
UniProt: View information about IL-15 on UniProt.org
Other Names: Interleukin 15, Interleukin-15, IL15, MGC9721