Biolegend, 571116, Cell-Vive™ GMP Recombinant Human IL-13 (carrier-free), 100μg

Initially cloned from cDNA libraries of activated T cells, human IL-13 is an immunoregulatory cytokine produced by Th2 type helper T cells in response to signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL 13 is a key mediator in the pathogenesis of allergic inflammation sharing many functional properties with IL-4 including the alpha subunit of the IL-4 receptor (IL-4Rα). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13 has been implicated in airway hypersensitivity, mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion. In vitro studies suggest IL-13 decreases SARS-CoV-2 entry to bronchial epithelial cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
100μg
Source: Human IL-13, amino acid (Gly21-Asn132) (Accession: # X69079) was expressed in E.coli.
Molecular Mass: The 112 amino acid recombinant protein has a predicted molecular mass of 12.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 9 kD by SDS-PAGE. The N-terminal amino acid is Gly.
N-terminalSequence Analysis: Gly-Pro-Val-Pro-Pro-Ser-Thr-Ala-Leu-Arg
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤0.500 ng/μg by ELISA
Concentration: 25 µg and 100 µg sizes are bottled at 0.5 mg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Human IL-13 induces proliferation of human TF-1 cells in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.0 ng/mL. The specific activity of Cell Vive™ GMP Recombinant Human IL-13 (carrier-free) is ≥ 2.8 x 105 IU/mg when compared against the WHO International Standard for human IL-13 (NIBSC code: 94/622).
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Monomer
Ligand/Receptor: IL-4Rα, IL-13Rα1, and IL-13Rα2
Bioactivity: Human IL-13 induces the proliferation of TF-1 cells
Cell Sources: IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils and NK cells
Cell Targets: IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells
Antigen References: McKenzie ANJ, et al. 1993. P Natl Acad Sci USA. 90:3735. Papasavvas E, et al. 2005. J. Immunol. 175:5532. Kelly-Wellch, et al. 2005. Science Signaling. 2005:293. Hershey GK. 2003. J Allergy Clin Immunol. 111:677. Harris J, et al. 2007. Immunity. 27:505. LaPorte SL, et al. 2008. Cell. 132:259. Jackson DJ, et al. 2020. J Allergy Clin Immunol. 146:203. e3. Bonser LR, et al. 2022. Am J Respir Cell Mol Biol. 66:391.
Regulation: IL-13 modulates human monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in human monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β
Gene ID: 3596
UniProt: View information about IL-13 on UniProt.org
Other Names: ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789, IL13