Biolegend, 572409, Recombinant Human GM-CSF (ELISA Std.), 4pack

GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain nonhemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro , although the in vivo significance of these observations remains uncertain.
4pack
Source: Human GM-CSF, amino acids Ala18- Glu144 (Accession# AAA52578) was expressed in E. coli, approximately 14.6 kD.
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application: ELISA
Recommended Usage: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This GM-CSF protein is useful as a standard for a human GM-CSF sandwich ELISA, using unlabelled anti-GM-CSF antibody, clone BVD2-23B6 (catalog #502202) for capture and biotinylated BVD2-21C11 (catalog #502304) antibody for detection.
Structure: Cytokine
Distribution: T cells, macrophages, fibroblasts, endothelial cells, mast cells.
Function: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Interaction: Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
Ligand/Receptor: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common
Bioactivity: Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines interleukin (IL)—3 and IL-5 regulate the production and functional activation of hemopoietic cells, with GM-CSF acting on monocyte/macrophages and all granulocytes. GM-C
Biology Area: Cell Biology, Stem Cells
Molecular Family: Growth Factors, Cytokines/Chemokines
Antigen References: 1. Hercus TR, et al. 2009 Blood 114:1289-1298. 2. Hayashida K, et al. 1990 Proc Natl Acad Sci USA. 87:9655-9659. 3. Walker F, et al. 1985 EMBO J 4:933-939. 4. Kitamura T, et al. 1989 J. Cell Physiol. 140:323.
Gene ID: 12981
UniProt: View information about GM-CSF on UniProt.org
Regulatory Status: RUO
Other Names: Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA).