Biolegend, 575809, Recombinant Mouse IL-10 (ELISA Std.), 4pack

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. Recombinant mouse IL-10 is a 18.5 kD protein containing 160 amino acids.
4pack
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application: ELISA
Recommended Usage: ELISA with capture antibody clone JES5-2A5 (Cat. No. 504901) or clone MK10A6 (Cat. No. 521801) and detection antibody clone JES5-16E3 (Cat. No. 505003), with an approximate standard range from 30 pg/mL to 4 ng/mL.
Product Citations: Vázquez-Zapién GJ, et al. 2022. Cells. 11: . PubMed
Structure: Acid-labile cytokine; dimer
Bioactivity: Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation i
Cell Sources: Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets: T cells, B cells, mast cells, macrophages
Receptors: IL-10R (CDw210)
Biology Area: Immunology, Cell Biology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314. 3. Howard M, et al. 1992. Immunol. Today. 13:198. 4. Quesniaux V. 1992. Research Immunol. 143:385.
Regulation: Downregulated by IL-4, IL-10
Gene ID: 16153
UniProt: View information about IL-10 on UniProt.org
Regulatory Status: RUO
Other Names: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)