Biolegend, 578616, Cell-Vive™ GMP Recombinant Human G-CSF (carrier-free), 100μg

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
100μg
Source: Human G-CSF, amino acids Thr27-Pro200 (Accession# BC033245.1) was expressed in 293E cells.
Molecular Mass: The 174 amino acid recombinant protein has a predicted molecular mass of 18.6 kD. The DTT-reduced and the non-reduced proteins migrate at approximately 20 kD by SDS-PAGE. The N-terminal amino acid is Thr.
N-terminalSequence Analysis: Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 µm filtered protein solution is in PBS.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤0.500 ng/μg by ELISA
Concentration: 500 µg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 0.02 - 0.08 ng/mL as measured by its ability to induce proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The specific activity of Cell Vive™ GMP Recombinant Human G-CSF (carrier-free) is ≥ 3.2 x 107 IU/mg when compared against the WHO International Standard for human G-CSF (NIBSC code: 09/136).
Application: Bioassay Cell cultures
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Structure: Dimer
Distribution: Hematopoietic stem and progenitors, embryonic stem cells
Function: G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxide anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction: Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell line
Ligand/Receptor: CD114 (G-CSFR)
Bioactivity: Measured by its ability to induce proliferation of M-NFS-60 cells
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Nagata S, et al. 1986. Nature. 319:415. Kim HK, et al. 2006. Blood. 108:812. Donahue RE, et al. 2009. Blood. 114:2530. Shimoji K, et al. 2010. Cell Stem Cell. 6:227. Martins A, et al. 2010. IUBMB Life. 62:611. Hara M, et al. 2011. J. Exp. Med. 208:715.
Gene ID: 1440
UniProt: View information about G-CSF on UniProt.org
Other Names: Granulocyte-Colony Stimulating Factor, CSF3, CSF-β