Biolegend, 580009, Recombinant Rat IFN-γ (ELISA Std.), 4pack

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
4pack
Source: Rat IFN-γ, amino acids Gln23-Cys156 (Accession# NM_138880) was expressed in E. Coli.
Molecular Mass: 16 kD
Purity: >98%
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application: ELISA - Quality tested
Recommended Usage: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 2000 pg/ml to 31 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This IFN-γ protein is useful as a standard for a rat IFN-γ sandwich ELISA, using unlabeled DB-1 antibody (Cat. No. 507801 or 507808) for capture and biotinylated Poly5188 antibody (Cat. No. 518803) for detection.
Structure: Cytokine, dimer, 40-80 kD (Mammalian)
Distribution: CD8+ and CD4+ T cells, NK cells
Function: Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
Gene ID: 25712
UniProt: View information about IFN-gamma on UniProt.org
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma