Biolegend, 581914, Cell-Vive™ GMP Recombinant Human IL-7 (carrier-free), 25μg

IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γ chain (CD132, γc) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. In addition, IL-7Rα is shared with TSLP. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Nevertheless, mutations in the α chain of the IL-7 receptor in patients with severe combined immunodeficiency (SCID) confirmed that IL-7 is indispensable for T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7.  Nevertheless, most recent information suggests that IL-7 dependence in human lymphopoiesis increases during the progression of ontogeny in cord blood and bone marrow. IL-7 can be associated to hepatocyte growth factor (HGFβ) to form a hybrid cytokine (IL-7/HGFβ), which induces greater proliferation of CFU-S, SLPs, and pre–pro-B cells than does native IL-7. The hybrid cytokine signals through both IL-7R (IL-7Rα plus γc) and c-Met. IL-7 has a thymic antiapoptotic effect and induces the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of proapoptotic proteins Bax and Bad. In addition, IL-7 is a key regulator of glucose uptake in T lymphocytes. TGF-β has been shown to down-regulate IL-7 mRNA and protein secretion from human bone marrow stromal cells. In addition, TGF-β inhibits IL-7-induced proliferation of pre-B cells. BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:
25μg
Source: Human IL-7, amino acids Asp26-His177 (Accession # NP_000871.1) was expressed in 293E cells.
Molecular Mass: The 165 amino acid recombinant protein has a predicted molecular mass of approximately 18.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 20 - 30 kD by SDS-PAGE. The C-terminal contains an 8His-(TG8HGGQ)-tag.
N-terminalSequence Analysis: Asp-(Cys)-Asp-Ile-Glu-Gly-Lys-Asp-Gly-Lys
Purity: ≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation: 0.1 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level: Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content: ≤ 0.500 ng/µg by ELISA
Concentration: 500 µg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 0.2 – 1.2 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with PHA-activated human peripheral blood lymphocytes (PBL). Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. The specific activity of Cell-Vive™ GMP Recombinant Human IL-7 (carrier-free) is >1 x 108 IU/mg when compared against the WHO International Standard for Human IL-7 (NIBSC code: 90/530).
Application: Bioassay Cell Culture
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Disclaimer: BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Distribution: IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells.
Function: IL-7 induces proliferation of human immature B cells, and it is critical for T-cell development and peripheral T-cell homeostasis. Regulates T folicular helper cells.
Interaction: B cells, Hematopoietic stem and progenitors
Ligand/Receptor: IL-7Rα (CD127), common gamma chain (γc or CD132)
Bioactivity: Measured by its ability to induce proliferation of 7TD1 cell
Cell Type: B cells, Dendritic cells, Epithelial cells, Leukemia, Lymphocytes, NK cells, T cells, Tfh, Thymocytes
Biology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Stem Cells
Molecular Family: Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References: Park LS, et al. 1990. J. Exp. Med. 171:1073. Fry TJ and Mackall CL. 2002. Blood 99:3892. Lai L, et al. 2006. Blood 107:1776. Link A, et al. 2007. Nat. Immunol. 8:1255. Wofford J, et al. 2008. Blood 111:2101. Parrish YK, et al. 2009. J. Immunol. 182:4255. Saini M, et al. 2009. Blood 113:5793. Guimond M, et al. 2009. Nat. Immunol. 10:149.
Gene ID: 3574
UniProt: View information about IL-7 on UniProt.org
Other Names: Interleukin 7, Lymphopoietin-1 (LP-1), IL7