Biolegend, 584555, Recombinant Frizzled-9-Fc Chimera (carrier-free), 50μg

Frizzled-9 (FZD9), a G-protein coupled receptor with seven transmembrane domains (7TM), is one of a 10 member Frizzled protein family. Frizzled proteins are principal receptors for the Wnt family of ligands, mediating canonical Wnt signaling. Frizzled proteins are present in most animals, but not present in plants or in simpler (single cell) eukaryotes. In the presence of Wnt ligand, Wnt binds to extracellular cysteine rich domain (CRD) of Frizzled. A receptor complex forms between Frizzled and low-density lipoprotein receptor-related protein 5/6 (LRP5/6). According to the identity of amino acid sequences, frizzled proteins can be divided into four subgroups: subfamily 1 (FZD1, FZD2, and FZD7), subfamily 2 (FZD5 and FZD8), subfamily 3 (FZD9 and FZD10), subfamily 4 (FZD4) and subfamily 5 (FZD3 and FZD 6). FZD9 is primarily expressed in the brain, eyes, skeletal muscles, testis, and kidneys. FZD9 is also expressed in neural precursor cells within the developing neural tube. FZD9 is a receptor for WNT2 and WNT7a. FZD9 gene resides within the common deletion region of chromosome 7 associated with Williams syndrome. Diseases linked to FZD9 include Williams-Beuren syndrome and serous adenocarcinoma of the fallopian tube. FZD9 is involved in the development of B-cells, the dentate gyrus, and bone.
50μg
Source: Recombinant human Frizzled-9, amino acid Leu23-Asp185 (Accession No. O00144), with a C-terminal human IgG1 (Pro100-Lys330) Fc, was expressed in 293E cells.
Molecular Mass: The 402 amino acid recombinant protein has a predicted molecular mass of approximately 44.6 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50 - 62 kD and 80 - 115 kD by SDS-PAGE respectively. The Nterminal amino acid is Leu.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in PBS, 10% Glycerol, pH 7.2
Endotoxin Level: Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration: 50 µg at 0.2 mg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 8 – 64 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by 20 ng/mL of recombinant mouse Wnt3a.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Monomer
Distribution: Expressed in the brain, eyes, skeletal muscles, testis, and kidneys
Function: Receptor for Wnt proteins that functions in the canonical Wnt/beta-catenin signaling pathway
Ligand/Receptor: Wnt2, Wnt7a
Bioactivity: Measured by its ability to inhibit Wnt3a activity in Topflash assay
Cell Type: Embryonic Stem Cells, Lymphocytes, Monocytes
Biology Area: Cell Biology, Immuno-Oncology, Immunology, Neuroscience, Signal Transduction, Stem Cells
Molecular Family: Fc Receptors, Soluble Receptors
Antigen References: Smith AJ, et al. 2022. Sci Rep. 12:2388. Zacarías-Fluck MF, et al. 2021. Life Sci Alliance. 4:e201900490. David R. 2011. Nat Rev Mol Cell Biol. 12:280. Tennis MA, et al. 2005. Neoplasia. 12:244-53. Sompel K, et al. 2022. Front Oncol. 12:815737. Ranheim EA, et al. 2005. Blood. 105:2487-94. Zhao C, et al. 2005. Development. 132:2917-27. Albers J, et al. 2011. J Cell Biol. 192:1057-72.
Gene ID: 8326
UniProt: View information about Frizzled-9 on UniProt.org
Regulatory Status: RUO
Other Names: Frizzled9, Frizzled-9, FZ-9, FZD9, fzE9, HFZ9, seven-transmembrane receptor frizzled-9, Wnt receptor, CD349 antigen, CD349
Q: Why choose BioLegend recombinant proteins?
A: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
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Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)