Biolegend, 596306, Recombinant Mouse CD130 (gp130)-Fc Chimera (carrier-free), 100μg

Gp130 (glycoprotein 130), also known as CD130, IL6ST (IL-6-receptor-associated signal transducer), or common β chain, is a type I transmembrane glycoprotein consisting of 597 amino acid (aa) extracellular domain, one Ig-like domain, five fibronectin type-III repeats, a short transmembrane domain, and 277 amino acids intracellular domain. CD130 is expressed on T cells, activated B cells, monocytes, plasma cells, endothelial, and epithelial cells. Gp130 is a common subunit shared by a variety of functional cytokine receptors within the IL-6 and IL-12 family, including IL-6, IL-11, leukemia inhibitory factor (LIF), Oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), novel neutrophin-1/B cell stimulating factor-3 or cardiotrophin-like cytokine (CLC), Neuropoietin (NP), IL-27, IL-35, and IL-39. Gp130 is utilized as the signal-transducing molecule by these receptor complexes for initiating signal transmission. Binding of IL-6 to IL-6 R (IL-11 R) induces gp130 homodimerization to form the high-binding monomeric four-helix bundle complex, which activates the intracellular Janus Kinase/Signal Transducer and Activator of Transcription (JAK/STAT) signaling pathways. Gp130 promotes mainly pro-inflammatory immune responses, prevents apoptosis and inhibits of regulatory T cell (Treg) differentiation, regulate hematopoiesis, and bone metabolism. It also has been shown to have a role in embryonic development and is essential for the survival of motor and sensory neurons and for the differentiation of astrocytes.
100μg
Source: Mouse gp130, amino acid (Gln23-Glu617) (Accession: # Q00560.2), with a linker (AAANSSLGS) and a C-terminal human IgG1 (Pro100-Lys330), followed by a 6His tag, was expressed in CHO cells.
Molecular Mass: The 841 amino acid recombinant protein has a predicted molecular mass of approximately 94 kD. The DTT-reduced and non-reduced proteins migrate at approximately 115 - 130 kD and 240 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level: Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration: 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Recombinant mouse gp130 inhibits the proliferation of mouse 7TD1 cells induced by recombinant human IL-6 in the presence of Recombinant Human IL-6Rα (Cat. No. 751502). The ED50 for this effect is 0.4 – 4 ng/mL.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Dimer
Distribution: T cells, activated B cells, plasma cells, and monocytes
Function: Signal transduction, regulate immune responses, apoptosis, Treg differentiation, hematopoiesis, and bone metabolism
Ligand/Receptor: IL-6RA, IL-11RA, IL-27RA, OSMR, LIFR, CNTFR, JAK1, and SOCS3
Bioactivity: Recombinant mouse gp130-Fc chimera inhibits the proliferation of mouse 7TD1 cells induced by recombinant human IL-6 in the presence of recombinant human IL-6Rα (Cat. No 751502).
Cell Type: B cells, Dendritic cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Narazaki, M, et al. 1993. Blood. 82:1120-6. Kishimoto T, et al. 1997. Annu Rev Immunol. 15:797-819. Hibi M, et al. 1990. Cell 63:1149-57. Kishimoto T, et al. 1995. Blood. 86:1243-54. Dominitzki S, et al. 2007. J Immunol. 179:2041-5. Atreya R, et al. 2000. Nat Med. 6:583-8. Ritter K, et al. 2020. Cells. 9:2695. Silver JS, et al. 2010. J Leukoc Biol. 88:1145-56.
Gene ID: 16195
UniProt: View information about CD130 on UniProt.org
Regulatory Status: RUO
Other Names: Glycoprotein 130, CD130, CD130 antigen, CDw130, Membraneglycoprotein 130, IL6ST, IL6-beta, IL-6RB, IL-6R-beta, IL-6R subunitbeta, IL-6 receptor subunit beta, interleukin-6 receptor subunitbeta, gp130 of the rheumatoid arthritis antigenic peptide-b
Q: Why choose BioLegend recombinant proteins?
A: • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.
Q: How does the activity of your recombinant proteins compare to competitors?
A: We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
Q: What is the specific activity or ED50 of my recombinant protein?
A: The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
Q: Have your recombinants been tested for stability?
A: Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
Q: Does specific activity of a recombinant protein vary between lots?
A: Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
Q: How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
A: Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)