Biolegend, 600302, Purified anti-MRP-14 (S100A9) Antibody, 100μg

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human S100A8/S100A9 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per mL (1:1000-1:10000). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize S100A8. The 114 amino acid recombinant S100A9 protein has a predicted Mw of ~13 kD for monomer and ~26 kD for dimer.
Product Citations: Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID: AB_2721746 (BioLegend Cat. No. 600301) AB_2721747 (BioLegend Cat. No. 600302)
Structure: Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8. S100A9 has 114 amino acids with the predicted Mw of ~13 kDa.
Distribution: MDSCs, neutrophils, monocyte, and ileal tissue eosinophils
Function: Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction: Calcium, S100A8
Ligand/Receptor: RAGE, TLR4
Cell Type: Neutrophils, Monocytes, Eosinophils
Biology Area: Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References: 1. Loser K, et al. 2010. Nat. Med. 16:713. 2. Cheng P, et al. 2008. J. Exp. Med. 205:2235. 3. Leukert N, et al. 2006. J. Mol. Biol. 359:961-72. 4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402. 5. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID: 6280
UniProt: View information about MRP-14 on UniProt.org
Clone: A15105J
Regulatory Status: RUO
Other Names: S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype: Mouse IgG2b, κ