Biolegend, 600351, Purified anti-Aquaporin-4 Antibody, 25μg

Aquaporin-4 (AQP4) is a member of the water-selective channel family that is predominantly expressed in astrocytes in the central nervous system. AQP4 is a regulator of water homeostasis by exchanging water across the endfoot membranes in the brain. This protein is vital for maintaining the normal level of cerebrospinal fluid influx into the brain cortex, parenchyma, and perivascular space.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human AQP4 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, IHC-F, WB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Ramadhanti J, et al. 2013. Monoclon Antib Immunogiagn Immunother. 32:270-276
RRID: AB_2904437 (BioLegend Cat. No. 600351) AB_2904437 (BioLegend Cat. No. 600352)
Structure: Aquaporin 4 is a 323 amino acid protein with a molecular mass of 35 kD.
Distribution: Tissue distribution: astrocytes in CNS Cellular distribution: plasma membrane, endosome, and cytoskeleton
Function: AQP4 facilitates the transport of water across the blood-brain interface.
Cell Type: Astrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References: Salman MM, et al. 2021. Nat Rev Neurosci. 22:650-651 Tang G and Yang GY. 2016. Int J Mol Sci. 17:1413 Jarius S and Wildemann B. 2013. Brain Pathol. 23:661-83
Gene ID: 361
UniProt: View information about Aquaporin-4 on UniProt.org
Clone: E5206
Regulatory Status: RUO
Other Names: MIWC, WCH4, AQP4
Isotype: Mouse IgG1, κ