Biolegend, 600658, PE anti-AMPKα Phospho (Thr172) Antibody, 100tests

AMP-activated protein kinase alpha (AMPKα) is one subunit of the αβγ heterotrimeric protein complex AMPK. It is a key regulator of metabolism and energy homeostasis in eukaryotes through induction of catabolic pathways and deactivation of anabolic. As an inhibitor of mammalian target of rapamycin complex 1 (mTORC1) pathway, AMPKα is involved in the regulation of cellular growth, cell cycle progression, autophagy, and may play dual roles as both tumor suppressor and promotor. AMPKα is activated via phosphorylation of Threonine residue 172 (Thr172) within the activation loop of the α subunit and is promoted by the allosteric binding of AMP and/or ADP to AMPK’s γ subunit. This phosphorylation is mediated by the serine/threonine kinase liver kinase B1 (LKB1) in conjunction with accessory subunits STRAD and MO25 and also by calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2 aka CAMKKβ). Activation is induced by increases in cellular AMP:ATP and ADP:ATP ratios  following a decline in ATP levels as well as by cellular stress, DNA damage, glucose starvation, and fluxes in calcium and nutrient levels. Localization of AMPKα Thr172 at the spindle poles suggests a novel role for AMPK in mitotic spindle orientation. AMPKα is a key therapeutic target in the treatment of metabolic disorders and an emerging potential treatment target for cancer.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human AMPK alpha phosphorylated at Thr172
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality testeed
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend TruePhos™ Perm Buffer (Cat. No. 425401) for fixation and permeabilization
RRID: AB_3699060 (BioLegend Cat. No. 600657) AB_3699060 (BioLegend Cat. No. 600658)
Structure: AMPKα is a 552 amino acid protein with a predicted molecular weight of 63 kD.
Distribution: Heart, Skeletal muscle, Kidney / Nucleus and cytoplasm
Function: Serine/threonine-protein kinase, transcription regulation, Wnt signaling, metabolism
Ligand/Receptor: ATP-binding, magnesium, Metal-binding, Nucelotide-binding
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Mihaylova MM, et al. 2011. Nat Cell Biol 13:1016-23. Stein SC, et al. 2000. Biochem J. 345:437-43. Thaiparambil JT, et al. 2012. Mol Cell Biol. 16:3203-17. Vara-Ciruelos D, et al. 2019. Open Biol. 9:190099. Willows R, et al. 2017. Biochem J. 474:3059-3073.
Gene ID: 55635562
UniProt: View information about AMPKalpha Phospho Thr172 on UniProt.org
Clone: A20017A
Regulatory Status: RUO
Other Names: PRKAA1, PRKAA2, AMPKa1, AMPKa2, AMPK, ACACA Kinase, Tau-Protein Kinase PRKAA1, Tau-Protein Kinase PRKAA2, Protein kinase AMP-Activated, Alpha 1 Catalytic Subunit, HMGCR Kinase
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.