Biolegend, 600754, Alexa Fluor® 488 anti-c-Fos Antibody, 100tests

The c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. C-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human FOS protein fragments
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal. For ICFC, we recommend fixation/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal.
RRID: AB_3097468 (BioLegend Cat. No. 600753) AB_3097468 (BioLegend Cat. No. 600754)
Structure: c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.
Distribution: Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes
Function: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
Interaction: Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
Antigen References: Dony C and Gruss P. 1987. Nature. 328:711-4. Grigoriadis AE, et al. 1994. Science. 266:443-8. Glover JN and Harrison SC. 1995. Nature. 373:257-61.
Gene ID: 2353
UniProt: View information about c-Fos on UniProt.org
Clone: 7D6-A9-D4
Regulatory Status: RUO
Other Names: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos
Isotype: Mouse IgG1, κ