Biolegend, 600852, Purified anti-FosB Antibody, 100μg

FosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full-length recombinant FosB protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICC, we recommend fixation in 4% PFA and permeabilization for 6 minutes in 0.5% Triton-X. Methanol only and PFA + Methanol fix/perm methods were tested and produced inferior signal.
RRID: AB_2922629 (BioLegend Cat. No. 600851) AB_2922629 (BioLegend Cat. No. 600852)
Structure: FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.
Distribution: FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.
Function: FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.
Interaction: Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G/C TCA) SWI/SNF chromatin remodeling complex, DPP4
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family: Tumor Suppressors
Antigen References: Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.
Gene ID: 2354
UniProt: View information about FosB on UniProt.org
Clone: W19011B
Regulatory Status: RUO
Other Names: G0/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B
Isotype: Rat IgG2a, κ