Biolegend, 601351, Purified anti-UCHL1 Antibody, 25microl

Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a thiol protease and member of the ubiquitin carboxyl-terminal hydrolase (UHC) family of deubiquitinating enzymes (DUBs). Highly expressed in the brain and neuroendocrine system, UCHL1 maintains long-term potentiation (LTP), regulates synaptic structure and function, plays a role in the protection of neuroendocrine cells, and is required for the structure and function of neuromuscular junctions. As an important component of the neuronal ubiquitin-proteasome pathway (UPP), UCHL1 tags aberrant proteins for proteasomal degradation, stabilizes and recycles free ubiquitin, regulates axonal transport, plays a critical role in axonal and neuronal integrity and repair, and may protect them from hypoxia-induced cell death following injury and ischemia. Loss or mutation of UCHL1 has been linked to axonal and dendritic pathology and subsequent deterioration of cognitive and motor functions and neurological disorders such as Alzheimer’s disease, Parkinson’s disease, and Amyotrophic lateral sclerosis, thus making it an important potential therapeutic target for such disorders. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Lines - UCHL1 (Horizon Cat. No. HZGHC003960c004)
25microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human UCHL1 protein
Formulation: PBS, 0.5% BSA, 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.1 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC, KO/KD - WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:8000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. For flow cytometric staining using our Cyto-Fast™ Fix/Perm Buffer Set, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: This clone was tested for ICC using HeLa cells (negative control) and Ramos cells (positive control) using three different fixation/permeabilization methods: methanol only, 4% PFA + methanol, and 4% PFA + Triton X-100. Each method produced nuclear and cytosolic staining in positive controls as well as cytosolic staining in negative controls. This clone failed to adequately enrich UCHL1 from Ramos whole cell extracts. For immunohistochemistry (IHC-P), Citrate Buffer, 10X (Cat. No. 420902) as antigen retrieval system is recommended.
RRID: AB_2904442 (BioLegend Cat. No. 601351)
Structure: UCHL1 is a 223 amino acid protein with an expected molecular weight of 27 kD.
Distribution: Expressed primarily in the central nervous system and gonads / Endoplasmic reticulum and cytoplasm
Function: Hydrolase, Thiol protease
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Ubiquitin/Protein Degradation
Antigen References: Bilguval K, et al. 2013. PNAS. 110:3489-94. Bishop P, et al. 2016. Biochem. J. 473:2453-62. Graham S and Liu H. 2017. Ageing Res. Rev. 34:30-38.
Gene ID: 7345
UniProt: View information about UCHL1 on UniProt.org
Clone: A19092E
Regulatory Status: RUO
Other Names: Ubiquitin carboxyl-terminal hydrolase 1, PGP9.5, Ubiquitin Carboxyl-Terminal Esterase L1, Neuron Cytoplasmic Protein 9.5, HEL-117, UCH-L1, HEL-S-53, NDGOA, PARK5, PGP95, SPG79
Isotype: Mouse IgG2b, κ