Biolegend, 601602, Purified anti-human Cytokeratin 7 Antibody, 100μg

Keratin 7 (KRT7) belongs to type II keratin and is expressed in the epithelia lining the cavities of internal organs, gland ducts and blood vessels. The function of KRT7 is still not clear but it has been suggested involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). In cancer, the expression of KRT7 is observed in many types of carcinoma and is associated with neoplasm progression. The diverse and unique expression pattern of KRT7 is often used as a diagnosis indicator in epithelial origin carcinoma. For example, aberrant KRT7 expression is observed in triple negative breast cancer. In bladder cancer, loss of KRT7 results in promoting proliferation of the bladder urothelium.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human KRT7 peptide (446-462) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250 - 1:2500 dilution). For immunocytochemistry, the recommended usage is 0.2 - 2.0 µg per mL (1:250 - 1:2500 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not react with mouse (in-house tested). Additional reported applications (for the relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed Zhang Y, et al. 2021. Front Oncol. 11:668707. PubMed
RRID: AB_2715904 (BioLegend Cat. No. 601601) AB_2715905 (BioLegend Cat. No. 601602)
Structure: Type II cytokeratin. 469 amino acids with a predicted molecular weight of 51 kD.
Distribution: Cytoplasm.
Function: KRT7 is a type II keratin, involved in the translational regulation of the human papillomavirus type 16 E7 mRNA.
Interaction: KRT7 interacts with EIF3S10, HPV16 E7, and GPER1.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Tajima Y, et al. 2017. Sci Rep. 7:40684. 2. Huang B, et al. 2016. Oncogene. 35:4927. 3. Szponar A, et al. 2012. Virchows Arch. 460:423. 4. Sano M, et al. 2010. Int J Oncol. 36:321.
Gene ID: 3855
UniProt: View information about Cytokeratin 7 on UniProt.org
Clone: W16155A
Regulatory Status: RUO
Other Names: Keratin type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Type-II keratin Kb7, Sarcolectin, SCL, Cytokeratin 7, Keratin 7
Isotype: Rat IgG2a, κ