Biolegend, 602352, Purified anti-CDK1 Antibody, 100μg

Cyclin-dependent kinase 1 (CDK1) is a serine/threonine kinase that is ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that universally induces entry into mitosis in eukaryotes. CDK1 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. In addition to regulation by protein-protein interactions, CDK1 is controlled by phosphorylation, particularly at conserved tyrosine residues. In humans, CDK1 is encoded by the gene CDC2 (cell division control protein homolog 2).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human CDK1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product was tested and did not produce a detectable signal for mouse cross-reactivity in WB. This product was tested and did not produce a detectable signal for Immunoprecipitation. This product was tested and did not produce a detectable signal in IHC in human skin tissue.
RRID: AB_2922631 (BioLegend Cat. No. 602351) AB_2922631 (BioLegend Cat. No. 602352)
Structure: CDK1 is a 297 amino acid protein with an estimated molecular weight of 34.1 kD.
Distribution: CDK1 is expressed throughout the cytoplasm and nucleus.
Function: CDK1 is a serine/threonine kinase.
Interaction: CDK1 phosphorylates a myriad of targets including transcription factors, other kinases, enzymes, and structural and nuclear proteins associated with mitosis. Additionally, CDK1 is regulated by other kinases, most notably Wee1, which inhibits CDK1 activity.
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Malumbres M. 2014. Genome Biol. 15:122. Draetta G, et al. 1988. Nature. 336:738-44.
Gene ID: 983
UniProt: View information about CDK1 on UniProt.org
Clone: A20004C
Regulatory Status: RUO
Other Names: Cell division cycle protein 2 (CDC2), G1 to S and G2 to M, cyclin dependent kinase 1 (CDK1), cdc28A, p34 kinase
Isotype: Mouse IgG2b, κ