Biolegend, 603452, Purified anti-Maf Antibody, 100μg

Transcription factor Maf (Maf) is a transcription factor encoded by the MAF gene. In a complex as a homo- or hetero-dimer, Maf acts as a transcriptional activator and repressor with diverse functions. For example, Maf plays numerous roles in the immune system by regulating IL-4 expression and modulating T-cell apoptosis, and is also a critical regulator of glucagon production in pancreatic alpha cells. Additionally, Maf plays a role in the development of multiple tissues, including the eye, bone, and blood vessels. Mutations in Maf are known to cause congenital and juvenile cataracts.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant N-terminus fragment of human Maf
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is 0.03 - 0.05 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone has been tested and does not work for IHC-P. For ICC application, paraformaldehyde plus Triton-X and paraformaldehyde plus methanol fix/perm methods are recommended. The use of methanol only as fix/perm is not suitable for this antibody. For ICFC application, Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended.
RRID: AB_2910482 (BioLegend Cat. No. 603451) AB_2910482 (BioLegend Cat. No. 603452)
Structure: Maf is a 373 amino-acid protein with a predicted molecular weight of 38.5 kD.
Distribution: Nucleus
Function: Transcription factor
Interaction: Maf has been shown to interact with CREBBP, EP300, MYB and SOX9.
Cell Type: Endothelial cells, Osteoblasts, T cells, Th2
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Transcription Factors
Antigen References: Imbratta C, et al. 2020. Front Immunol. 11:206. Zhang C and Guo ZM. 2015. Diabetes Metab Res Rev. 31:773-8.
Gene ID: 4094
UniProt: View information about Maf on UniProt.org
Clone: W18010A
Regulatory Status: RUO
Other Names: Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, T Lymphocyte C-Maf Long Form
Isotype: Rat IgG2a, κ