Biolegend, 603552, Purified anti-OGT Antibody, 100μg

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) catalyzes the transfer of N-acetyl glucosamine (GlcNAc) post-translational modification to serine, threonine, and cysteine residues of proteins. Serine and threonine residues are modified by O-glycosidic linkage, while cysteine is modified by S-glycosidic linkage. Since the amino acids utilized for O-GlcNAcylation are identical to those used for protein phosphorylation, OGT competes with kinases for modification of those sites. Numerous functions for GlcNAcylation have been reported in regulating protein-protein interactions, and OGT plays diverse roles in the regulation of the cell cycle, transcription, and cellular stress. Dysregulation of O-GlcNAcylation plays a role in the pathology of Alzheimer’s disease, cancer and diabetes. OGT activity is regulated by glucose availability, and plays a role in regulating glycolysis by glycosylation of phosphofructokinase.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human OGT protein fragments
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product does not meet passing criteria for ICC and IHC.
RRID: AB_2922635 (BioLegend Cat. No. 603551) AB_2922635 (BioLegend Cat. No. 603552)
Structure: OGT is a 1,046 amino acid protein with a predicted molecular mass of 116.9 kD.
Distribution: OGT is expressed in the cytoplasm of most cells.
Function: OGT is an enzyme which catalyzes the attachment of the O-GlcNAc post-translational modification.
Interaction: OGT glycoylates a wide array of proteins including histone H2B, AKT1, and EZH2.
Biology Area: Cell Cycle/DNA Replication, Cell Proliferation and Viability, Protein Synthesis
Molecular Family: Enzymes and Regulators, Protein Modifiers
Antigen References: Canovas B and Nebreda A. 2021. Nat Rev Mol Cell Biol. 22:346-366.
Gene ID: 8473
UniProt: View information about OGT on UniProt.org
Clone: W20094A
Regulatory Status: RUO
Other Names: O-linked N-acetylglucosaminyltransferase (O-GlcNAc transferase, OGT) subunit p110, UDP-N-acetylglocuosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Isotype: Rat IgG2b, κ