Biolegend, 603702, Purified anti-STAT1 Antibody, 100μg

STAT1, also known as signal transducter and activator of transcription 1, is a ubiquitously expressed protein that is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, cytosolic STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcriptional activator. Two isoforms of STAT1, with predicted molecular weights of 87 (STAT1α) and 83 kD (STAT1β), exist as a result of alternative RNA processing. This clone is specific for STAT1α. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human STAT1 peptide phosphorylated at Serine 727.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 µg per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is specific for STAT1 isoform a and is not predicted to recognize the ß isoform due to the absence of the region corresponding to the immunizing peptide.This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).U3A cells were selected for antibody specificity testing because the cell line does not express STAT11.This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole.
Application References(PubMed link indicates BioLegend citation): Muller M, et al. 1993. EMBO J. 12:4221
Product Citations: Kiritsy MC, et al. 2021. Elife. 10:. PubMed
RRID: AB_2749867 (BioLegend Cat. No. 603701) AB_2749868 (BioLegend Cat. No. 603702)
Structure: STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.
Distribution: Ubiquitously expressed; cytosolic and nuclear distribution
Function: Transcription activator/Cytokine Signaling
Interaction: Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area: Cell Biology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Durbin JE, et al. 1996. Cell 8:443. Darnell JE Jr, et al. 1994. Science 264:1415. Chen X, et al. 1998. Cell 93:827. Ramana CV, et al. 2000. Oncogene 19:2619.
Gene ID: 6772
UniProt: View information about STAT1 on UniProt.org
Clone: A15158C
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 1, Transcription factor ISGF-3 components p91/p84
Isotype: Mouse IgG1, κ