Biolegend, 604551, Purified anti-COMT Antibody, 25μg

Catechol-O-methyltransferase (COMT) catalyzes the addition of methyl groups to catecholamine hormones and neurotransmitters; in particular: dopamine, norepinephrine, and epinephrine. Norepinephrine and epinephrine are key mediators of the sympathetic nervous system, while dopamine is an important neurotransmitter in the regulation of mood and behavior. Since O-methylation inactivates target catecholamines and leads to its degradation, COMT activity has significant implications for human health. The Val158Met polymorphism of the COMT gene is associated with mental disorders such as schizophrenia, obsessive compulsive disorder, bipolar disorder, and substance dependence.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human COMT
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. For intracellular flow cytometric staining, the suggested concentration range of this reagent is 0.01 - 0.03 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Product is not recommended for Western Blot. For ICC, recommend fixation/permeabilization with 100% ice-cold methanol. Do not recommend fixation with 4% PFA.
RRID: AB_2922640 (BioLegend Cat. No. 604551) AB_2922640 (BioLegend Cat. No. 604552)
Structure: COMT is a 271 amino acid protein with a predicted molecular weight of 30.0 kD.
Distribution: COMT can be found in the cytosol and on the plasma membrane.
Function: Methyltransferase
Cell Type: Dopaminergic Neurons, Mature Neurons, Neurons
Biology Area: Neurodegeneration, Neuroscience, Neuroscience Cell Markers
Molecular Family: Enzymes and Regulators
Antigen References: Akhtar MJ, et al. 2020. Bioorg Chem. 94:103418. Scheggia D, et al. 2012. CNS Neurol Disord Drug Targets. 11:209-21. Hosak L. 2007. Eur Psychiatry. 22:276-81.
Gene ID: 1312
UniProt: View information about COMT on UniProt.org
Clone: A17180E
Regulatory Status: RUO
Other Names: Catechol-O-methyltransferase (COMT), Epididymis Secretory Sperm Binding Protein Li 98n, Testicular Tissue Protein Li 42, EC 2.1.1.6, HEL-S-98n
Isotype: Mouse IgG1, κ