Biolegend, 604851, Purified anti-XPC Antibody, 25μg

XPC is a protein involved in the nucleotide excision repair (NER) pathway, a mechanism that repairs single-stranded DNA damage. XPC is a member of the XPC complex that recognizes DNA damage resulting in thermodynamically unstable DNA structures. For example, the unstable structures caused by the damage from ultraviolet light. XPC-RAD23B activation recruits other NER pathway members to the site and promotes progression through NER. XPC mutations can result in the disease that is the protein’s namesake, Xeroderma pigmentosum, characterized by an extreme sensitivity to sunlight and high cancer risk. More broadly speaking, since DNA damage underlies many types of cancer, changes in XPC expression levels or mutations in XPC are often found in tumors.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human XPC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product does not cross-react with mouse by WB. This product is not recommended for ICC and IHC-P.
RRID: AB_2924610 (BioLegend Cat. No. 604851) AB_2924610 (BioLegend Cat. No. 604852)
Structure: XPC is a 940 amino-acid protein with a predicted molecular weight of 106 kD.
Distribution: XPC is expressed in most mammalian cells, shuttling between the nucleus and cytoplasm in the absence of DNA damage.
Function: XPC binds DNA in the presence of DNA damage
Interaction: XPC forms the XPC complex along with RAD23B and CETN2
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family: Nuclear Markers
Antigen References: Nezow L, et al. 2015. DNA Repair. 26:19-27. Sugasawa K, 2008. Adv Exp Med Biol. 637:47-56.
Gene ID: 7508
UniProt: View information about XPC1 on UniProt.org
Clone: W20162C
Regulatory Status: RUO
Other Names: DNA repair protein complementing XP-C cells (XPC), Xeroderma pigmentosum complementation group C (XPC), p125, XPC-Complex Subunit
Isotype: Rat IgG2b, λ