Biolegend, 605051, Purified anti-APEX1 Antibody, 25μg

Apurinic-apyridimic endonuclease-1, or APEX1, is a multifunctional enzyme required for the repair of DNA damaged by both endogenous and exogenous agents. It also exerts oncogenic function as a redox signaling protein by reductively stimulating the DNA-binding activity of transcription factors that drive cancer progression, including c-Jun, AP-1, NF- κ B, p53, and HIF1α. APEX1 expression level and subcellular localization are positively correlated with cancer progression, and elevated APEX1 expression may also confer resistance to DNA-damaging chemotherapeutic agents.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full-length recombinant human APEX1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining. When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.
RRID: AB_2910484 (BioLegend Cat. No. 605051) AB_2910484 (BioLegend Cat. No. 605052)
Structure: APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.
Distribution: Nucleus/Ubiquitously expressed
Function: DNA repair enzyme
Biology Area: Cell Biology, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Antigen References: Fishel ML, et al. 2008. DNA Repair. 7:177-86. Kim MH, et al. 2013. J Clin Invest. 123:3211-3230. Thakur, et al. 2014. Exp Mol Med. 46:e106.
Gene ID: 328
UniProt: View information about APEX1 on UniProt.org
Clone: 1518CT337.123.86.269.232
Regulatory Status: RUO
Other Names: Apurinic/Apyrimidinic Endodeoxyribonuclease 1, REF1, HAP1, APEN, APE, APX, AP Endonuclease 1
Isotype: Mouse IgG1, κ