Biolegend, 605552, Purified anti-Jun Phospho (Ser73) Antibody, 100μg

c-Jun is the first oncogenic transcription factor ever discovered. It heterodimerizes with members of the Fos protein family, such as c-Fos, to form the AP-1 transcription factor complex, exhibiting both activating and repressive functions depending on the interacting partner and promoter sequence context. c-Jun is required for cell cycle progression, and is protective against DNA damage, such as that resulting from ultraviolet light exposure. c-Jun is also overexpressed in many cancers including lung, breast and vulvar cancer, and is a target for anti-cancer therapies. Phosphorylation of c-Jun at serine 63 and 73 is activational, and mediated by the MAPK signaling cascade.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human c-Jun phosphorylated at Ser73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 3.0 - 5.0 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2924613 (BioLegend Cat. No. 605551) AB_2924613 (BioLegend Cat. No. 605552)
Structure: c-Jun is a 331 amino acid protein with a predicted molecular weight of 35.7 kD.
Distribution: c-Jun is primarily expressed in the nucleus
Function: Transcription factor
Interaction: c-Jun forms the AP-1 transcription factor complex by heterodimerizing with proteins of the Fos family
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Pulverer B, et al. 1991. Nature. 353:670-4. Vogt P. 2001.Oncogene. 20:2365-77 . Brennan A, et al. 2020. J Exp Clin Cancer Res. 39:184.
Gene ID: 3725
UniProt: View information about Jun Phospho Ser73 on UniProt.org
Clone: A19012A/R/C
Regulatory Status: RUO
Other Names: Transcription factor Jun, activator protein 1 (AP1), Proto-oncogene c-Jun, transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Isotype: Mouse IgG1, κ