Biolegend, 605554, Alexa Fluor® 488 anti-Jun Phospho (Ser73) Antibody, 100tests

c-Jun is the first oncogenic transcription factor ever discovered. It heterodimerizes with members of the Fos protein family, such as c-Fos, to form the AP-1 transcription factor complex, exhibiting both activating and repressive functions depending on the interacting partner and promoter sequence context. c-Jun is required for cell cycle progression, and is protective against DNA damage, such as that resulting from ultraviolet light exposure. c-Jun is also overexpressed in many cancers including lung, breast and vulvar cancer, and is a target for anti-cancer therapies. Phosphorylation of c-Jun at serine 63 and 73 is activational, and mediated by the MAPK signaling cascade.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human c-Jun phosphorylated at Ser73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3097470 (BioLegend Cat. No. 605553) AB_3097470 (BioLegend Cat. No. 605554)
Structure: c-Jun is a 331 amino acid protein with a predicted molecular weight of 35.7 kD.
Distribution: c-Jun is primarily expressed in the nucleus
Function: Transcription factor
Interaction: c-Jun forms the AP-1 transcription factor complex by heterodimerizing with proteins of the Fos family
Biology Area: Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Pulverer B, et al. 1991. Nature. 353:670-4. Vogt P. 2001.Oncogene. 20:2365-77 . Brennan A, et al. 2020. J Exp Clin Cancer Res. 39:184.
Gene ID: 3725
UniProt: View information about Jun Phospho Ser73 on UniProt.org
Clone: A19012A/R/C
Regulatory Status: RUO
Other Names: Transcription factor Jun, activator protein 1 (AP1), Proto-oncogene c-Jun, transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39
Isotype: Mouse IgG1, κ