Biolegend, 605752, Purified anti-NSE Antibody, 100μg

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human NSE
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 - 10.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2924615 (BioLegend Cat. No. 605751) AB_2924615 (BioLegend Cat. No. 605752)
Structure: Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution: Tissue distribution: Nervous and musculoskeletal systems. Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.
Function: Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type: Mature Neurons, Neurons
Biology Area: Neuroscience, Neuroscience Cell Markers
Molecular Family: Enzymes and Regulators
Antigen References: Murray GI, et al. 1993. J Clin Pathol. 46:993-6. Duncan ME, et al. 1992. J immunol Methods. 151:2227-36.
Gene ID: 2026
UniProt: View information about NSE on UniProt.org
Clone: A18254F
Regulatory Status: RUO
Other Names: Gamma-enolase, neural enolase, neuron-specific enolase (NSE), enolase 2 (ENO2), 2-phospho-D-glycerate hydrolyase
Isotype: Mouse IgG2b, λ