Biolegend, 605953, Ultra-LEAF™ Purified anti-human B7-H6 Antibody, 100μg

B7-H6 is a type I transmembrane protein and member of the B7 family. It contains 454 amino acids with a predicted molecular weight of 51 kD and has several potential N-glycosylation sites. Similar to the other members of the B7 family, B7-H6 possesses two Ig like domains in the extracellular domain. The most related proteins to B7-H6 are B7-H1 and B7-H3. B7-H6 is expressed in human cancer cells such as myeloid and lymphoblastic leukemia, neuroblastomas, and melanomas, and it binds to NKp30 to activate human NK cells and target cell killing. Nevertheless, tumor cells can escape NK-cell-mediated recognition. A mechanism of immune escape involves tumor cell shedding of B7-H6. That process is mediated by ADAM10 and ADAM17. Thus, soluble B7-H6 high levels in blood have been detected in patients with malignant melanoma; levels that correlate with elevated B7-H6 expression in melanoma biopsies. In addition, B7-H6 is expressed in glioma stem-like cells derived from glioma cell lines in vitro. Soluble isoforms form of B7-H6 have also been detected in serum during normal pregnancy. These isoforms, of ∼37kD and ∼30kD, maintain the C- and N-terminus, and it seems that they are released by a mechanism different from proteolytic cleavage. B7-H6 can be induced in healthy cells by inflammatory process. B7-H6 and NKp30 orthologues do not exist in mice.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human B7-H6
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 1 µg/mL biotinylated recombinant human NKp30 to 1 µg/mL immobilized recombinant human B7-H6 (Cat. No. 794006). ND50 range: 0.01 - 0.08 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927840 (BioLegend Cat. No. 605953) AB_2927840 (BioLegend Cat. No. 605954)
Structure: Dimer
Distribution: Human tumor cell lines, cancer cells, monocytes and neutrophils after inflammatory stimulation, glioma stem-like cells. Not detected in healthy cells.
Function: Natural killer (NK) cell activation and cytotoxicity
Interaction: NK cells
Ligand/Receptor: NKp30 (CD337)
Cell Type: Leukemia, Monocytes, Neutrophils
Biology Area: Cancer Biomarkers, Cell Death, Immuno-Oncology, Immunology
Antigen References: Brant CS, et al. 2009. J Exp Med. 206:1495-503. Kaifu T, et al. 2011. Cell Mol Life Sci. 68:3531-9. Matta J, et al. 2013. Blood. 122:394-404. Schlecker E, et al. 2014. Cancer Res. 74:3429-40. Chen Y, et al. 2018. Path Oncology Res. 24:717-21. Gutierrez-Franco J, et al. 2018. Immunobiology. 223:57-63. Obiedat A, et al. 2020. J Mol Med. 98:135-48. Chen H, et al. 2020. J Immunol Res. 2020:2328675.
Gene ID: 374383
UniProt: View information about B7-H6 on UniProt.org
Clone: W20205G
Regulatory Status: RUO
Other Names: B7H6, NCR3LG1, natural killer cell cytotoxicity receptor 3 ligand 1, B7 Homolog 6
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.