Biolegend, 607001, Purified anti-Spt16 (FACT140 complex) Antibody, 25μg

Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant (full length)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg per ml is recommended. For ChIP applications, the suggested dilution is 1:50 - 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: 25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution. ICC positive in HeLa Cells.
Application References(PubMed link indicates BioLegend citation): Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271 Adelmant G, et al. 2012. Mol Cell Proteomics. 11:411. PubMed Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
Product Citations: Goswami I, et al. 2022. EMBO Rep. 23:e53684. PubMed Burmistrova D, et al. 2016. PLoS One. 11: 0150958. PubMed Fleyshman D, et al. 2017. Oncotarget. 8:20525. PubMed Adelmant G, et al. 2012. Mol Cell Proteomics. 11:411. PubMed Isa NF, et al. 2021. Vaccines (Basel). 9:. PubMed Safina A, et al. 2017. Nucleic Acids Res. 10.1093/nar/gkw1366. PubMed Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed Hu X, et al. 2019. Front Genet. 10:684. PubMed Kaja A, et al. 2021. Mol Cell Biol. 41:. PubMed Lolis A, et al. 2013. J Biol Chem. 288:7676. PubMed Lin D, et al. 2018. EMBO J. 37. PubMed Sandlesh P, et al. 2018. PLoS One. 15:81. PubMed Prendergast L, et al. 2020. Oncogene. 39:5124. PubMed
RRID: AB_315688 (BioLegend Cat. No. 607001) AB_315689 (BioLegend Cat. No. 607002)
Structure: 120-140 kD
Distribution: Nuclear
Function: Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation
Interaction: Component of FACT ATP-dependent chromatin remodeling complex, SSRP1 protein in FACT complex, H2A/H2B dimers
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. LeRoy G, et al. 1998. Science 282:1836. 2. Orphanides G, et al. 1998. Cell 92:105. 3. Orphanides G, et al. 1999. Nature 400:284.
Gene ID: 11198
UniProt: View information about Spt16 FACT140 complex on UniProt.org
Clone: 8D2
Regulatory Status: RUO
Other Names: Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68
Isotype: Mouse IgG2a, κ