Biolegend, 607251, Purified anti-MALT1 Antibody, 25μg

MALT1 is a ubiquitously expressed protease that is localized in the cytoplasm as a catalytically inactive BCL10-bound proenzyme. MALT1 acts downstream of oligomeric CARMA/CARD-BCL10-MALT1 (CBM) complexes to regulate various functions in which MALT1 serves a dual purpose. Firstly, MALT1 is a scaffolding protein that plays an integral role in the regulation of the NF-κB and MAP kinase p38 pathways. In addition to serving as a scaffolding protein, MALT1 also possesses protease activity which promotes lymphocyte activation by cleavage of signaling proteins. Furthermore, MALT1 possesses a caspase-like domain with two conserved residues, cysteine 464 and histidine 415, which act as catalytic mechanisms in caspases and cysteine proteases ultimately contributing to inflammation, cell death, and cell proliferation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human MALT-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For ICC, we recommend fixation with 4%PFA, followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol. We do not recommend methanol fixation. This antibody is not suitable for WB due to poor target selectivity.
RRID: AB_2927965 (BioLegend Cat. No. 607251) AB_2927965 (BioLegend Cat. No. 607252)
Structure: MALT1 is an 824 amino-acid protein with a predicted molecular weight of 92 kD.
Distribution: MALT1 is ubiquitously expressed in the cytoplasm.
Function: Scaffolding protein, protease
Interaction: MALT1 acts downstream of CARMA/CARD-BCL10-MALT1 (CBM) complexes to regulate NF-kappa-B and MAP kinase p38 pathways.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability
Molecular Family: Enzymes and Regulators, Proteases, Scaffold Proteins
Antigen References: Beyaert R, et al. 2015. FEBS J. 282:3286-97. Thome M, et al. 2018. Front Immunol. 9:1927. Hailfinger S, et al. 2016. J Invest Dermatol. 136:788-79.
Gene ID: 10892
UniProt: View information about MALT1 on UniProt.org
Clone: W20127A
Regulatory Status: RUO
Other Names: MALT1 Paracaspase, PCASP1, MLT, Mucosa-Associated Lymphoid Tissue Lymphoma Translocation Protein 1, Mucosa Associated Lymphoid Tissue Lymphoma Translocation Gene 1, MALT1 Protease, MALT Lymphoma-Associated Translocation, Caspase-Like Protein, MLT1
Isotype: Rat IgG2b, κ